Differentiation of Lymphoid Cells: The Use of Specific Antisera to Characterize the Cells Required for the Induction of Immunoglobulin M Production in Vitro

[摘要] Anti-immunoglobulin as well as anti-thymocyte sera prevented the induction of immunoglobulin M production by rabbit popliteal lymph node cells that ordinarily occurs when such cells are cultured in vitro, in the absence of added antigen. Antibodies specific for the light chains and µ-chains of rabbit immunoglobulins were inhibitory whereas sera specific for α- and γ-chains were without effect. Univalent anti-µ-chain antibody and univalent anti-thymocyte antibody preparations were also inhibitory. All inhibitory antisera were without effect when added to cultures after the induction process was completed. Thymidine incorporation studies showed a lack of correspondence between the inhibitory activity and mitogenicity of anti-immunoglobulins.

[学科分类] 生物科学（综合）