Receptors for Antigen on Lymphoid Cells

[摘要] A method was developed for examining the binding specificities of plaque-forming cells (PFC) which were immune to either goat red blood cell surface antigens or to the trinitrophenyl (TNP) haptenic group. Immune rat lymph node cells were plated on poly-l-lysine fixed red blood cell (RBC) monolayers which functioned dually as immunoadsorbents and as plaque indicators of both adherent and nonadherent PFC populations. Anti-TNP PFC demonstrated marked specific adherence to TNP-GRBC. The adherence was optimal following a 30-min adsorption phase and was essentially independent of the adsorption temperature in the 4° to 37°C range. Specific adherence of anti-GRBC PFC to GRBC monolayers at 4°C was not detectable, but did occur at adsorption temperatures of 25° or 37°C. Temperature differences in specific adsorption in the two systems were not due simply to differences in the density of antigenic determinants on the adsorbing monolayers. Specific adherence of anti-GRBC PFC at 4°C was able to occur in the presence of DEAE-dextran, indicating the importance of surface charge effects. This immunoadsorbent-indicator system provides a means of studying the recognition events occurring at the PFC surface.

[学科分类] 生物科学（综合）