Plaque Assay for the Detection of Alloantibody-Producing Cells in Mice

[摘要] A method was developed for the detection of alloantibody plaque-forming cells (PFC) in the mouse based on the use of red blood cells as indicator cells and of normal rabbit serum as the source of complement. Up to 510 PFC per spleen (34 PFC per 107 spleen cells) were detected in primary reactions involving the combination of strains B10.A to C57BL/10 which differ at the H-2 locus and up to 1610 PFC per spleen (115 PFC per 107 spleen cells) in secondary reactions involving the combination A to C57BL/6 which differ at both H-2 and non-H-2 loci. In the latter combination, the assay was shown to detect both cells producing antibodies to H-2 and non-H-2 antigens. The antigens determined by the H-2D region induced the formation of larger numbers of plaques than the antigens determined by the H-2 K region.

[学科分类] 生物科学（综合）