Active Immunity to Influenza Virus in the Mouse

[摘要] It has now been repeatedly shown that it is possible to produce a considerable degree of immunity to the intranasal inoculation of influenza virus in both mouse and ferret by the parenteral injection of living or inactivated influenza virus. This communication is an account of experiments which have been carried out in order to investigate the specificity of the immunity produced and the effect which inactivation has on the specificity of the immunizing antigens.Experimental Methods. Preparation of viral suspensions. All suspensions were prepared in the following way. Swiss mice were inoculated intranasally under ether-anesthesia with a heavy suspension of virus and were killed 48 hours later. Irrespective of whether there were pulmonary lesions or not, 5 per cent suspensions by weight were made by grinding the lungs in equal parts of broth and saline solution with a little sand. The suspensions were then clarified by centrifugation for 30 minutes in the more horizontal position of an angle centrifuge.

[学科分类] 生物科学（综合）