Influence of Collodion Particles on the Visible End-Point in Antibody Titrations

[摘要] 1. 1. Collodion particles combined with autolysates of pneumococcus, meningococcus and gonococcus were agglutinated when the suspensions were mixed with antibacterial sera. The titers obtained in this manner were much greater than could be obtained by comparable mixtures of organisms and antisera, whole organisms, collodion particles and antisera, or autolysates and antisera.2. 2. There is apparently an optimal concentration of collodion particles requisite for obtaining maximal titers from antisera.3. 3. Titers obtained in non-specific reactions were lower than those obtained by specific mixtures of autolysates, collodion particles and antisera.4. 4. It was found by experiment that the most satisfactory titers were obtained by allowing collodion particles to remain suspended in autolysate. It is suggested that no washing of the particles be made to free them of “excess” antigen in view of the fact that antigenic substances other than adsorbable proteins might be present.5. 5. The phenomenon of increased titer effected by use of collodion particle-autolysate antigens was observed with both low- and high-titered sera.

[学科分类] 生物科学（综合）