Retention of Rabbit Antibody Activity during Acetylation

[摘要] Although acetylation destroys the ability of rabbit anti-BSA to form a precipitate with BSA it was found here that it still combines with BSA to form soluble complexes. The combination was studied by electrophoretic and ultracentrifugal methods. Acetylation of 96% of the amino groups of anti-BSA γ-globulin resulted in a preparation that could still form soluble complexes with BSA. Normal rabbit γ-globulin, however, when acetylated to 86% of its amino groups, did not form soluble complexes with BSA.The failure of acetylated antibody to form a precipitate with its antigen is attributed to electrostatic repulsion effects between antibody molecules.

[学科分类] 生物科学（综合）