Fluorescent Microscopic Study of Sendai Incomplete Virus Production in Tissue Culture Cells

[摘要] When monkey kidney cells were infected with 1 EID50 of Sendai virus per cell, infectious virus was produced and the cytoplasmic inclusions could be observed by the fluorescent antibody indirect method. Antigen was never seen in the nuclei.Diffuse fluorescence of the cytoplasm without cytoplasmic inclusions could be seen after inoculation with 100 EID50 per cell. Here, presumably incomplete virue production with a low EID50:HA ratio had taken place.Incomplete Sendai virus was also formed in monkey kidney tissue culture in the presence of proflavine. The cytopathic effect appeared earlier and was more intense than in controls. The fluorescent antibody indirect technique detected the cells as large, structureless brightly fluorescing globi with a high concentration of antigen in the cytoplasm as well as in the nuclei. Acridine-orange staining showed profound changes in the nucleus and the lack of RNA in newly-formed antigen. The possible role of the nucleus in incomplete virus production is supposed.

[学科分类] 生物科学（综合）