A New Model to Study Hematopoietic Transplantation Antigens

[摘要] A method for the study of hematopoietic transplantation antigens is described. It seems to fulfill the requirements for an immunochemical bioassay, i.e. , to be simple, sensitive and quantitative. The procedure is as follows: a) Recipient mice are primed with varying doses of homologous donor antigens, and donors with sheep RBC antigen. b) Ten days later the recipients are exposed to 800 r and injected intravenously with 24 × 106 spleen cells of the donor, followed by intraperitoneal injection of sheep RBC. c) Anti-sheep RBC titer is determined 6 days later and compared with the titer of nonprimed controls.The RD50 (the dose of priming donor antigen capable of stimulating the host to reject 50% of the donor spleen cells) is 6 × 102 for viable bone marrow cells, 4 × 104 for bone marrow cells killed with x-rays, 1 × 105 for liver cells, and 5 × 105 for testicular cells. No priming potency was found with even 107 RBC.

[学科分类] 生物科学（综合）