Antigenic Cross-Reactivity between 7 S and 19 S Rabbit γ-Globulin

[摘要] Immunoelectrophoretic analyses of rabbit γ-globulin preparations containing varying amounts of the 19 S fraction have shown that 19 S and 7 S γ-globulin form cross-reacting arcs of different electrophoretic mobility. Pure preparations of 19 S γ-globulin were also shown to cross-react with 7 S γ-globulin in Ouchterlony plates. With an appropriate antiserum both proteins demonstrate spurs, indicating individual as well as common antigenicity.Absorption of a sheep antiserum to 7 S γ-globulin with fragment III (papain digestion) does not affect the line formed with the 19 S fraction but removes the spur formed by the 7 S fraction.Absorption of this antiserum with fragments I and II removes the 19 S are but leaves the 7 S line. This demonstrates that 7 S and 19 S rabbit γ-globulin share some of the antigenic groups associated with the antibody carrying fragments of the 7 S molecule.

[学科分类] 生物科学（综合）