Physicochemical and Immunologic Properties of Eluates Containing I-125 Anti-Rh0(D)

[摘要] 1. 1 . The effect of different elution procedures on the physicochemical properties and the immunologic behavior of I-125 anti-Rh(D) containing eluates derived from the same antiserum was studied.2. 2 . Differences in the properties of the I-125 anti-Rh(D) eluates could be attributed to the composition of the globulin containing fraction used for sensitization, the physical form of the antigen-containing red cells used for absorption-elution, the manner in which the red cells were converted to stroma and the modality used for eluting the sensitized stroma. Eluates were prepared by using heat to 56°C, an acid medium to pH 3.5 and by lipid solvent disruption of the red cell membrane.3. 3 . The I-125 anti-Rh(D) eluates showed differences in antibody content, rate of association with red cells and in their reaction with red cells in antibody excess (zoning).4. 4 . Zoning was observed in eluates prepared by heat from red cells or stroma sensitized with a crude globulin fraction, and in eluates prepared from sensitized cells lysed with digitonin and eluted by acid.5. 5 . All I-125 anti-Rh(D) eluates on immunoelectrophoresis contained γG-globulins of extended (fast) electrophoretic mobility. The increase in the anionic charge of the eluted I-125 anti-Rh(D) is probably due to the presence of soluble antigen-antibody complexes in the eluates.6. 6 . When a homogeneous γG fraction was used for sensitization of red cells, it was possible to prepare a heat and an acid eluate which did not differ significantly from each other in either physicochemical properties or immunologic behavior.

[学科分类] 生物科学（综合）