Studies on Human Peripheral Blood Lymphocytes in Vitro:

[摘要] 1. 1 ) Pokeweed mitogen (PWM)-stimulated cells show maximum transformation after 72 hr in culture. When optimal concentrations of PWM are used, the transformed cells do not exceed 50 to 60% of the total.2. 2 ) After 72 hr the population of cells includes small and medium-size lymphocytes, large phytohemagglutinin (PHA)-like blast cells and a unique intermediate sized cell type.3. 3 ) The intermediate sized PWM cell is characterized by an eccentric nucleus with clumped heterochromatin, a well developed Golgi apparatus, ribosomal aggregates and a well developed rough-surfaced endoplasmic reticulum. The fine structural features differ importantly from those consistently seen in PHA transformed cells.4. 4 ) The possible immunologic significance of PWM stimulation of human peripheral lymphocytes in vitro has been considered.

[学科分类] 生物科学（综合）