A Sensitive Hemagglutination Assay Method for Dinitrophenyl-Specific Antibodies

[摘要] A sensitive and specific passive hemagglutination (HA) assay for 2,4-dinitrophenyl (DNP)-specific antibodies is described. Red blood cells (RBC) are sensitized by coupling DNP groups to their surfaces. The sensitivity of the method was obtained by the use of “optimally sensitized” RBC and a serum diluent containing dextran and fetal calf serum. The method detects as little as 0.005 to 0.010 µg/ml of anti-DNP antibody protein. The method was found to be about three times more sensitive for antibodies with relatively high affinity ( Ko , 1010 L/mole), than for those with low affinity ( Ko , 106 L/mole).

[学科分类] 生物科学（综合）