PANCREATIC ENZYMES: NORMAL OUTPUT AND COMPARISON OF DIFFERENT METHODS OF ASSAY

[摘要] Serial analyses of duodenal juices of 47 children were made by parallel estimations with different methods of amylase, trypsin and lipase assay for studying normal conditions and to establish a common denominator which would, independently from the method of estimation used and from the enzyme in question, indicate the normal or pathologic character of the findings. On the basis of more than 1,200 determinations it is proposed to take the lowest values recorded as 100% and to express any value below these figures as their fractions in per cent.No differences of importance could be found between the enzyme values of different age groups and there were certainly no variations in the lipase levels.No periodicity could be observed in the output of enzymes. Fluctuations occurred but these showed no periodicity and it could be proved that they were due to dilution of the pancreatic juice by bile. Fasting secretion continuously occurred in these healthy children and the amount of enzymes put out was constant in units of time.A strict parallelism in the output of the three enzymes was observed in all the present cases. This holds true also following stimulation by pilocarpine and inhibition by atropine and whatever variation occurred happened simultaneously with each enzyme.Six children were given unbalanced diets to determine whether the production of enzymes is adapted to the food given. The changes found during and after the diets could by no means be connected with the type of food eaten.It is emphasized that all the above data were found in healthy and normal children and that the conditions described are thoroughly different in most pathologic conditions.

[学科分类] 儿科学