Retroactivity to the Output of Transcription Devices: Quantification and Insulation.

[摘要] Traditional engineering often relies on hierarchical design techniques to build com-plex systems from simpler subsystems. This technique requires modularity, a prop-erty that states that the input/output characteristics of a system are not affected byinterconnections. In this work we investigate retroactivity, an impedance-like effectin biomolecular systems that makes the behavior of a system change upon intercon-nection. We show, through analysis and experiments, that retroactivity in syntheticbiology circuits is responsible for substantial changes in a system dynamic response.In order to construct circuits modularly, we propose the design of insulation de-vices, which, similar to insulating amplifiers in electronics, attenuate retroactivityeffects and recover modular behavior. Our technique is based on a novel disturbanceattenuation approach based on singular perturbation theory.

[发布机构] University of Michigan

[效力级别] Control Systems

[关键词] Synthetic Biology;Control Systems;Singular Perturbation;Systems Biology;Biomedical Engineering;Electrical Engineering;Biological Chemistry;Microbiology and Immunology;Molecular;Cellular and Developmental Biology;Engineering;Science;Electrical Engineering: Systems