Non-Random Sister Chromatid Segregation During Stem Cell Division in Drosophila Melanogaster Testis.

[摘要] Adult stem cells undergo asymmetric cell division to self-renew and to produce differentiated cells throughout the life of an organism. This increases the risk of replicative senescence or neoplastic transformation due to mutations that accumulate over many rounds of DNA replication. The immortal strand hypothesis proposes that stem cells reduce the accumulation of replication-induced mutations by retaining all the older template DNA strands. In addition, other models have also been proposed in which stem cells non-randomly segregate only a subset of chromosomes for different reasons, such as retention of epigenetic memories. However, the mechanism and the biological relevance of these chromosome asymmetries remain elusive. The Drosophila melanogaster testis is one of the few well-characterized model systems that enable a detailed study of the regulation of stem cells. To elaborate, unlike many other model systems Drosophila male germline stem cells (GSCs) can be unambiguously identified at single-cell resolution. Further, GSCs divide asymmetrically giving rise to a stem cell and a differentiating cell, which can be readily identified in vivo enabling unambiguous identification of both asymmetric stem cell division and any other potential asymmetries such as nonrandom sister chromatid segregation.In this thesis, I describe work where I first showed that the bulk of chromosomes are not segregated asymmetrically in dividing Drosophila GSCs, suggesting that GSCs do not retain all the older template DNA strands to maintain their genomic integrity. However, these initial results did not exclude the possibility that GSCs might be non-randomly segregating individual chromosomes. In order to unambiguously study the segregation patterns of individual chromosomes, I adapted the CO-FISH (chromosome orientation fluorescence in situ hybridization) protocol, which allows strand-specific identification of sister chromatids. Using this method, I found that sister chromatids of X and Y chromosomes, but not autosomes, are segregated non-randomly during asymmetric divisions of GSCs. These results provide the first direct evidence that sister chromatids of certain chromosomes can be segregated non-randomly during asymmetric stem cell divisions. Further, in this work I also showed that centrosomal proteins, nuclear envelope proteins, and methyltransferase are all required for non-random sister chromatid segregation of X and Y chromosomes.

[发布机构] University of Michigan

[效力级别] Drosophila Male Germline Stem Cell

[关键词] Non-random Sister Chromatid Segregation;Drosophila Male Germline Stem Cell;Molecular;Cellular and Developmental Biology;Health Sciences;Cell and Developmental Biology