Simultaneous Quantification of Glycyrrhetinic acid & Apigenin using HPTLC from Glycyrrhiza glabra Linn.

[摘要] Glycyrrhiza glabra Linn. commonly known as Mulethi was employed medicinally as an expectorant and carminative in ancient times. There are no reports of simultaneous quantification of Glycyrrhetinic acid & Apigenin from this plant. Hence a TLC densitometric method was developed and validated for quantification of these marker compounds. Glycyrrhetinic acid & Apigenin were quantified from 2N methanolic-HCl extract & methanolic extract respectively using the Solvent System of Ethyl Acetate: Ethanol: Water: Ammonia (6.3: 2: 0.4: 0.1 v/v). The method was validated using ICH guidelines in terms of precision, repeatability and accuracy. Linearity range for Glycyrrhetinic acid & Apigenin was found to be 160 to 960 ng/spot & 32 to 96 ng/spot respectively; and the content of Glycyrrhetinic acid & Apigenin was found to be 0.65 ± 0.059% & 0.0074 ± 0.0004% respectively. This simple, precise and accurate method gave good resolution from other constituents of extract.

[学科分类] 分析化学

[关键词] Ivabradine HCL;Pharmaceutical Dosage Form;RP-HPLC. Spectrophotometric;UV detector