Speciation of Organotin Compounds with Ion Pair-Reversed Phase Chromatography Technique

[摘要] The usage of ion-pair reversed phase Chromatography (IP-RP) technique for speciation of dibutyltin (DBT), tributyltin (TBT), and triphenyltin (TPhT) has been studied. These three species were able to separate on an ion pair-reversed phase chromatographic column. The eluats were detected on line by use hydride generation-quartz furnace atomic absorption spectrophotometry (HG-QFAAS) method. The eluent consisted of a mixture of methanol : water : acetic acid with a composition of 80 : 19 : 1, containing of 1 mM decane sulfonate acid as ion pairing reagent. The pH of the eluent was adjusted with 1 M H₂SO₄. The separation of the all of the species at above conditions was good performance, which was shown by the values of fundamental chromatographic parameters. The capacity factor (k') for DBT, TBT and TPhT species were 0.27, 2.54 and 5.92 respectively. The resolution (Rs) values for DBT-TBT and TBT-TPhT separation were 2.92 and 2.42 respectively, while the selectivity for DBT-TBT and TBT-TPhT were 9.76 and 3.50 respectively. These data show the effectiveness of developed chromatographic system.

[学科分类] 分析化学

[关键词] Spectrophotometry; Diazotization; Nitrite; Nitrate