A Stability-Indicating HPLC Method for Cefoperazone

[摘要] Stability indicating HPLC assay method for the standard drug cefoperazone was developed using reverse phase appurassil C-18, 250 X 4.6mm. 5µm column, in the mobile phase phosphate buffer (pH 6.8) and methanol (5:2) at flow rate 1mLmin-1 with UV detection at 254 nm. The retention time was found to be 2.67 min. Validation of an analytical method was established by laboratory studies. The proposed method was found to be linear at concentration of 1 to 10 µgmL-1 (R2=0.9899). The limit of detection and limit of quantification was 0.2 µgmL-1 and 0.4 µgmL-1 respectively and the method was found to be specific with respect to specificity. Method precision and precision of the system was found to be within the limits of the acceptance criteria. Relative Standard deviation for precision of the method and precision of the system was found to be 0.49% and 0.7451% respectively. The percentage recovery ranges from 95 –106 % The results indicate that there is no interference from excipients for the proposed method, thus making the method more simple, less time consuming and suitable for routine quantitative estimation ofcefoperazone sodium injection formulation. As the method could effectively separate the drug from its degradation products, it can be employed as a stability indicating one.

[学科分类] 分析化学

[关键词] Losartan Potassium; Hydrochlorothiazide; UV Spectrophotometry; Stability Indicating