Application of Fe(II)-5-Pyridyl Benzodiazepin-2-ones to the Determination of Plasma or Serum Glucose

[摘要] A new coupled redox procedure has been developed for the determination of glucose in 4 µl of plasma or serum. The photometric endpoint is formation of the violet-blue Fe2+-5-pyridyl benzodiazepin-2-one chelate, for which the absorbance at 580 nm is linear with glucose concentration from 25 to 300 mg/100 ml. Results obtained by the present technique and those obtained by the o -toluidine method and a glucose oxidase method agree well. The mean recovery of glucose added to plasma is 96.6% (range: 92.1-99.6%).

[学科分类] 过敏症与临床免疫学