Blood Glucose Determination without Deproteinization, with Use of o-Toluidine in Dilute Acetic Acid

[摘要] A method is described for determining glucose in 25 µl of plasma. o -Toluidine is used in weak acetic acid solution. Addition of the emulsifier "Cremophor" obviates the need for deproteinization. The reaction is complete after 15 min at 100°C, and the color is stable for longer than 24 h. The blank is almost colorless. The reagent is stable, colorless, not viscous, and convenient to handle.

[学科分类] 过敏症与临床免疫学