Aminoglycoside Interference in the Pyrogallol Red-Molybdate Protein Assay Is Increased by the Addition of Sodium Dodecyl Sulfate to the Dye Reagent

[摘要] The pyrogallol red-molybdate (PRM) protein dye-binding assay (1) is commonly used for urinary protein determination (2). The addition of sodium dodecyl sulfate (SDS) to the dye reagent is recommended to improve the uniformity of response to different proteins (3). The assay is prone to interference from aminoglycoside antibiotics, particularly gentamicin, neomycin, tobramycin, paromomycin, geneticin, and kanamycin (1)(2)(4)(5), but the degree of interference varies with different PRM methods. Thus, the DADE Behring PRM and the Sigma PRM assays are sensitive to aminoglycoside interference (4)(5), but the Cobas Fara and Roche Integra 700 PRM assays are not (4). The present study indicates that the addition of SDS to the dye reagent increases the susceptibility of the PRM assay to aminoglycoside interference. Thus, the concentration of SDS in commercial PRM reagents may be a contributory factor in determining …

[学科分类] 过敏症与临床免疫学