Screening for Interference in Immunoassays

[摘要] In their report in a recent issue of Clinical Chemistry , Emerson et al. (1) screened clinical samples for the presence of interference in four immunoassays. Three different techniques were applied, defining interference as (a) nonlinear assay responses with serial dilution, (b) discrepant assay results after pretreatment with heterophile blocking reagent (HBR), or (c) positive reactions on a mouse-antibody-negative control reaction (Tandem ICON® ImmunoConcentration hCG). The percentage of interference-positive samples varied significantly by technique, and the authors therefore concluded that prescreening for interfering substances with these assays is not warranted.False-positive results caused by assay interference could be detrimental if undetected, as shown in the hallmark report by Rotmensch and Cole (2). Interferences have been characterized or labeled as heterophile antibodies, human-anti-mammalian antibodies, human-anti-mouse (monoclonal) antibodies, …

[学科分类] 过敏症与临床免疫学