Recovery of Uric Acid from Serum

[摘要] Uric acid added to serum is incompletely recovered in tungstic acid filtrates of serum. Contrary to previous views, the poor recovery is not the result of the adsorption of uric acid upon the precipitated protein, nor is it the result of the presence in the filtrates of inhibitors of the color-forming reaction between the sodium carbonate and phosphotungstic acid reagents, and the uric acid. Evidence is presented which implicates adsorption on filter paper as the cause of the poor recovery of uric acid from acid filtrates. Excellent recoveries are obtained when the protein precipitated by tungstic acid is removed by centrifugation.

[学科分类] 过敏症与临床免疫学