Comparison of Atomic Absorption Spectrophotometry and Fluorometry for the Measurement of Serum Calcium

[摘要] An atomic absorption spectrophotometric (AAS) and a fluorometric method for the measurement of serum calcium were found to be comparable in acceptable reproducibility. The recovery figures obtained by the fluorometric procedure were considerably lower than those given by AAS in the 2-4 mEq./L. range, although recoveries at higher levels (6 mEq./L.) were acceptable. The average recovery for the fluorometric method throughout the range 2-6 mEq./L. was 83.8%; for AAS, 98.9%. Marked interference was observed in the fluorometric method when hemolyzed or icteric serum was assayed. In four groups of hospital patients—premature infants, newborn infants, older children, and adults—AAS gave ranges for serum calcium values which were higher than those obtained by fluorometry.

[学科分类] 过敏症与临床免疫学