Determination of Urinary Constituents in Solutions by Least-Squares Resolution of Ultraviolet Spectrums

[摘要] Least-squares resolution of ultraviolet (UV) spectrums has been tested as a method for determining the concentration of each UV-absorbing urinary component in a multicomponent solution. The tests reported here involved solutions containing 4-12 components chosen from 17 reference materials: p-aminobenzoic, anthranilic, ascorbic, ferulic, hippuric, p-hydroxybenzoic, uric, vanillic, and xanthurenic acids, and adenine, creatinine, cytosine, ergothioneine, hypoxanthine, indican, N-methylnicotinamide, and tryptophan. Spectrums of these reference and multicomponent solutions were measured from 1900 to 4000 Â, rather than over the more usual range of 2200 to 4000 Â; concentrations of individual components in unbuffered water solutions were in the range 0.4-100 mg./L. While the maximum number of solutes capable of being analyzed by this method cannot be given, this brief study shows that as many as 10 UV-absorbing urinary components could be determined quantitatively by least-squares resolution of the spectrum of the solution. All of the 17 species used in this study showed absorption in the region 1900-2200 Â; measurements in this region contributed greatly to the accuracy with which concentrations could be calculated.

[学科分类] 过敏症与临床免疫学