Effect of Different Sample Types and Stability after Blood Collection of N-Terminal Pro-B-Type Natriuretic Peptide as Measured with Roche Elecsys System

[摘要] N-Terminal pro-B-type natriuretic peptide (NT-proBNP; 76-amino acid peptide) is cosecreted with BNP and can be measured by automated immunoassay (Elecsys 2010; Roche UK). This is a fully automated electrochemiluminescence “sandwich” immunoassay based on capture (biotinylated) and detection (ruthenium) polyclonal antibodies against NT-proBNP amino acids 1–21 and 39–50 (1), respectively. Reports on in vitro stability are conflicting (2)(3)(4). We assessed the effect of different sample types on measurement of NT-proBNP and evaluated the effect of delayed separation of serum from cells on stability. We compared plasma in lithium heparinate (14 IU/mL of blood; Greiner Labortechnik; cat no. 9045-22-1) and serum in serum separator (SST) tubes from patients attending a heart failure clinic. For sample type comparison, blood from 60 patients was collected into SST and lithium heparin tubes. Blood was also collected in plain glass and SST …

[学科分类] 过敏症与临床免疫学