Serum Lipase Determination with an Olive Oil Substrate Using a Three-Hour Incubation Period

[摘要] The effect of various emulsifying agents, p H, buffers, and accelerators on the Cherry-Crandall procedure for determining serum lipase was studied. A method is proposed which uses a 3-hour incubation period. Olive oil emulsified with 7% acacia is the substrate. This is buffered with tris buffer, p H 8.0; magnesium acetate and EDTA are added as accelerators. Other conditions which result in an increased rate of hydrolysis are discussed.

[学科分类] 过敏症与临床免疫学