X-Ray Spectrometry in the Clinical Laboratory

[摘要] A procedure is described for estimating the bromide content of human serum from individuals not receiving bromide medication. In the procedure, the serum is ashed and dissolved in hydrochloric acid. The solution is dehydrated with the calculated amount of acetic anhydride and the precipitated sodium chloride is washed with additional glacial acetic acid to remove the bromides. The sodium chloride is removed by centrifugation and the bromides recovered by evaporation of the acetic acid. The residue is dissolved in methanol and transferred to filter paper for assay in the X-ray spectrometer. The procedure, with the omission of ashing, is applicable to sea water. When it was applied to sea water, strontium as well as bromide could be visualized on the recorder of the instrument and assayed.Normal human serum was found to contain a mean level of 252 µg. bromide per 100ml., with a range (2σ) of ± 42 µg./100 ml.

[学科分类] 过敏症与临床免疫学