Blood pH Measurement with the Glass Electrode

[摘要] A study has been described to explain the tendency of fingertip blood from normal individuals to drift to alkaline p H more rapidly than the blood of venous serum at 37°. This occurs even under oil and without loss of CO2. Evidence is offered to indicate that this phenomenon is due to the release of carbonic anhydrase in greater concentrations into the serum when the finger is punctured than when venipuncture is performed. An explanation is offered for the fact that serum from patients in acidemia with low CO2. content will not drift at 37°.It is suggested that blood p H determinations be made by collecting the specimen under oil, cooling rapidly to room temperature, and performing the determination under oil on the separated serum.

[学科分类] 过敏症与临床免疫学