Azorubin in Aqueous and Methanolic Solutions

[摘要] A study of the spectrophotometric characteristics of azorubin in varying proportions of methanol and water has been presented.The absorbence of azorubin in water was found to vary from 73 to 90 per cent of that in methanol, over a concentration range of 0.001 to 0.025 mg. per 2 ml. of test solution.The absorbence of azorubin was found to be linear over the same range of concentration, and over the range from 0.1 to 10 mg. per 100 ml. of serum, using a final test dilution of 1 to 8.The strong probability of the artefactual nature of "direct" and "total" serum bilirubin values has been demonstrated.

[学科分类] 过敏症与临床免疫学