Serum Protein Analysis by Electrophoresis and by the Wolfson-Cohn Chemical Method

[摘要] One hundred serum specimens from healthy and diseased adults were analyzed for the major protein constituents by free electrophoresis and by the Wolfson-Cohn chemical method. Albumin values obtained by the two methods were well correlated and were also in good average numerical agreement. The γ-globulin values were well correlated, but were not in quite such good numerical agreement, on a percentage basis, as were the albumin values. The α-globulin values were poorly correlated, as were also the β-globulin values.Electrophoretic examination of some of the chemical fractions indicated that part of the discrepancy between the two methods was caused by faulty chemical fractionation, particularly with respect to the globulin fractions.Determination of total solids retained by a dialysis membrane (TS) in 22 serum specimens confirmed the proportionality between TS and the electrophoretic-pattern area, and emphasized a source of error of undetermined magnitude in the calculation of electrophoretic results from simple protein totals.

[学科分类] 过敏症与临床免疫学