Automated Fluorometric Determination of Glucose-6-Phosphate Dehydrogenase (G6PD) and 6-Phosphogluconate Dehydrogenase (6PGD) Activities in Red Blood Cells

[摘要] An automated fluorometric microtechnic, suitable for screening purposes, has been developed for the quantitative determination of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) levels in red blood cells. The method is rapid, simple, and precise. It uses 50 µl whole-blood (capillary) samples and is based on the measurement of natural fluorescence emitted by the NADPH generated during the enzymatic reactions.

[学科分类] 过敏症与临床免疫学