Measurement of Cholesterol in Tissues with Phospho-6-tungstic-12-molybdic Acid

[摘要] A new spectrophotometric method for the determination of minute amounts of cholesterol in tissues is described. This method is a modification of the Yoshimatsu reaction which is based on the reduction of the yellow aqueous solution of phospho-6-tungstic-12-molybdic acid by a solution of cholesterol in concentrated sulfuric acid. The color of the resulting hydrosol of "molybdenum/tungsten blue" is particularly intense. The conditions for saponification confer virtual specificity for cholesterol. The proposed method is verified by comparison with estimates obtained by Abell’s procedure ( 9 ) as well as by the automated methodology of Block et al ( 8 ).

[学科分类] 过敏症与临床免疫学