Agar Gel Lipoprotein Electrophoresis: A Correlated Study with Ultracentrifugation

[摘要] Interest in lipoprotein electrophoresis has been stimulated by a reappraisal of hyperlipoproteinemic syndromes and definition of five basic types according to Fredrickson (Types I, II, III, IV, V). The basic analytic reference for lipoprotein studies remains ultracentrifugal analysis. We have adapted an agar gel lipoprotein electrophoretic system employing a photometric scan and quantification of lipoprotein fractions based on post-electrophoresis Sudan Black B staining. This method of lipoprotein partitioning has been compared with lipoprotein analysis by ultracentrifugation. Statistically significant correlation between the methods is demonstrated.

[学科分类] 过敏症与临床免疫学