Automated Fluorometry as Applied to the Assay of Individual Estrogens in Purified Urinary Extracts

[摘要] Using the Kober-lttrich fluorescence procedure, automated fluorometry was applied to the assay of individual estrogens and their 3-methyl ether derivatives in the purified urinary extracts of nonpregnant women. The values obtained by using this new automated scheme were compared with those obtained by the manual time-decay modification of the Slaunwhite-Engel fluorescence method; the comparison shows excellent correlation. A ratio of the corrected peak reading at 546 mµ. excitation to the corrected peak reading at 490 mµ. excitation was used not only to follow the effect of introducing additional purification stages in the development of assay procedures, but also to follow impurity levels in sample analyses. The same assembly of modules can be used for samples dissolved in acidified water, 70% methanol, and, with a simple change of manifold, in 1 N sodium hydroxide. This fully automated assay provides precise conditions in all stages.

[学科分类] 过敏症与临床免疫学