Liquid-chromatographic measurement of urinary monosaccharides.

[摘要] We describe "high-performance" liquid chromatography of urinary monosaccharides on a macroporous anion-exchange resin (Hitachi 3013N), with acetonitrile/water as eluent. The separated xylose, arabinose, D-fructose, mannose, galactose, and D-glucose were detected by means of a post-column reduction reaction of tetrazolium blue. Absolute limits of detection were about 10 ng of monosaccharide. Lactose can also be measured.

[学科分类] 过敏症与临床免疫学