Variation of pH of Clinical Samples as a Source of Error in Enzyme Determinations

[摘要] Variation of p H of urine and serum samples, either physiologic or that resulting from change on standing, may be a significant source of error in determination of enzyme activity. Buffer systems employed to control p H may be inadequate to cope with the variations which may be encountered. It has been demonstrated that such is the case in the determination of serum alkaline phosphatase by the method of Shinowara, Jones and Reinhart.

[学科分类] 过敏症与临床免疫学