Evaluation of a single-color-reading method for determining fructosamine.

[摘要] A commercial kit for determining fructosamine was evaluated. The reference interval (determined from data on 183 nondiabetic subjects) was 1.67 to 2.85 (mean 2.17) mmol/L. Serum and plasma (EDTA- or heparin-anticoagulated) gave equivalent results; plasma treated with fluoride/oxalate gave slightly lower values. The between-run CV was less than 4%. Fructosamine values were similar by the present method and the kinetic method. The standard curve was linear in the range of 1.3 to 8.5 mmol/L. None of several constituents of blood that we tested appreciably interfered. Fructosamine values were increased in some lipemic samples from non-diabetics, and were significantly correlated with glycated hemoglobin as measured by affinity chromatography. This kit evidently is a suitable alternative to the kinetic method for determining fructosamine.

[学科分类] 过敏症与临床免疫学