Microdetermination of Proline in Serum

[摘要] An accurate method for determination of proline in serum has been adapted to a micro scale, with good results. Acidic ethanol is used as a serum deproteinizing agent prior to development of color with ninhydrin and its extraction into benzene. The range of accuracy is ± 3.0% with as little as 0.2 ml. serum. The microdetermination of this amino acid facilitates study of the intermediary metabolism of proline in pediatric patients as well as in small laboratory animals. The method is also useful in the study of amino acid and collagen biosynthesis in a number of experimental systems.

[学科分类] 过敏症与临床免疫学