A Simple Method for the Quantitative Determination of N-Acetyl-p-Aminophenol (APAP) in Urine

[摘要] A simple method is described for the rapid quantitative analysis of N-acetyl- p -amino-phenol (APAP) in urine. APAP and its conjugates present in the urine following the ingestion of acetophenetidin, APAP or acetanilid, are hydrolyzed with acid to p -aminophenol. This compound is coupled with phenol in the presence of hypobromite to form an indophenol dye whose concentration is determined spectrophotometrically. Application of this method to a study of 3 human subjects treated with acetophenetidin is described.

[学科分类] 过敏症与临床免疫学