A New Method for the Determination of Ascorbic Acid in Urine

[摘要] A simple method for the determination of ascorbic acid in urine was developed. The method is based on the quantitative reduction of mercuric chloride to mercurous chloride at pH 3.5-5.0. The insoluble mercurous chloride was separated by centrifugation, dissolved in excess standard iodine-potassium iodide solution and back-titrated with standard sodium thiosulphate. The method has precision, and as little as 1.0 mg. of ascorbic acid per 100 ml. of urine can be determined with an accuracy of ± 3%. Common substances found in urine do not interfere.

[学科分类] 过敏症与临床免疫学