The Estimation of Galactose in Plasma Using Galactose Oxidase

[摘要] The estimation of galactose in plasma by the enzyme system galactose oxidase-peroxidase was investigated. The method was found to be relatively specific for galactose although xylose and ascorbic acid reacted to a slight degree with the enzyme; lactose also reacted with the enzyme, probably because partial hydrolysis of the disaccharide released galactose. The relationship between absorbance and concentration of galactose greater than 20 mg./100 ml. was linear. Below this value the line curved toward the origin, making accuracy uncertain unless a number of aqueous standards can be run with each batch of estimations.

[学科分类] 过敏症与临床免疫学