Determination of Free and Total Indole-3-acetic Acid and of the Indole Index

[摘要] A new method is described for measurement of the urinary excretion of free and total indole-3-acetic acid and for obtaining the chromogenic value of the total indoles. The color reaction employed is Fischl's (1) tryptophan reaction. Hydrolysis and extractions were done by Weissbach's (2) procedure. The ratio of the various fractions is expressed by a new term: "indoles index" (I.I.). By the evaluation of the data obtained under various conditions, many abnormalities of tryptophan metabolism may be detected.

[学科分类] 过敏症与临床免疫学