A Photometric System for Automatically Reading Concentration

[摘要] Spectrophotometric or colorimetric data are normally obtained in some type of photometric unit and must be dealt with mathematically to provide a final result expressed in concentration. Such mathematical operations are time-consuming, require data transfer, and are subject to human error. A photometric system has been devised which can be adjusted to perform calculations automatically and provide direct readout of concentration. This system combines logarithmic conversion of %T with control of both m and b constants in the straight-line equation, y = mx + b, in which y is absorbance, m is slope, x is concentration, and b is the blank correction. The system also provides linearizing compensation for those reactions which do not follow the Beer-Lambert law. The calibration procedure for the system is described, as is the linearization of nonlinear standard curves.

[学科分类] 过敏症与临床免疫学