A Modified Procedure for the Estimation of Total 17-Hydroxycorticosteroids (Porter-Silber Chromogens) in Urine

[摘要] A modified procedure for the determination of urinary 17-hydroxycorticosteroids (di-hydroxy-acetonyl-corticoids) is described. The use of ethyl ether in a single extraction step and the stabilization of the phenylhydrazine-sulfuric acid reagent with sodium chloride are the unique features of the method. Studies of chromogenicities of steroids, recovery, pH effect on extraction, and salt effect on the stability of Porter-Silber reagent in the presence and absence of light during storage are reported; chemical aspects of chromogenic development of various steroids are discussed, clinical ranges compared, and interpretations evaluated.

[学科分类] 过敏症与临床免疫学