Determination of Cholesterol by Means of Phloroglucinol in Acid Solution

[摘要] Cholesterol is determined by taking advantage of the fact that the digitonin moiety of cholesteryl digitonide is a glycoside containing in its molecular structure one molecule of xylose and four molecules of galactose, and therefore reacts with a sugar reagent, such as phloroglucinol in acid solution, to form a stable color complex yielding quantitative results when measured spectrophotometrically. The method agrees well with results obtained by the Schoenheimer and Sperry (8) procedure.

[学科分类] 过敏症与临床免疫学