Fluorometric Determination of Urinary Metanephrine and Normetanephrine

[摘要] A detailed procedure for the quantitative determination of metanephrine and normetanephrine is given. Epinephrine and norepinephrine are eliminated by selective adsorption on an alumina column. Metanephrine and normetanephrine are isolated with a cation-exchange resin. Fluorescence is developed by a two-step ferricyanide oxidation at two different pH values followed by tautomerization. The concentrations of both metanephrine and normetanephrine are quantitated by differential fluorometry.

[学科分类] 过敏症与临床免疫学