Solid-phase enzyme immunoassay for hepatitis B surface antigen.
[摘要] A solid-phase enzyme immunoassay is described for measuring hepatitis B surface antigen in human serum or plasma. Immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. In the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. Results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specificity.
[发布日期] [发布机构]
[效力级别] [学科分类] 过敏症与临床免疫学
[关键词] [时效性]