Advantages of Use of Ascorbic Acid and Sodium Lauryl Sulfate in the Microdetermination of Serum the Inorganic Phosphorus

[摘要] Vol. 27p 639 : In the second paragraph of the middle column, the third sentence ( et seq .) should read as follows: In the method of Chen et al. ( 12 ), modified to include SLS 1,040 mmol/L, 25 µL of serum was added to 25 µL of distilled water. To this was added 200 µL of a solution of SLS containing 1040 mmol/L, followed, after mixing, by 2.50 mL of a reagent consisting of 3.1 g of ammonium molybdate per liter of 375 mmol/L sulfuric acid. To avoid any instability in the reducing capacity of ascorbic acid if mixed in advance with molybdate and sulfuric acid ( 14 ), we added it separately—0.200 mL of a 150 g/L solution in glass-distilled water. After incubation in a warm bath at 37 °C for 140 min ( 12 ), the replicated stoppered samples were cooled and their absorbances (820 nm, 1-cm cells) read immediately and 3 and 18 hlater. In those cases where SLS was not used—i.e., in the unmodified method ( 12 )—50 µL of a prepared trichloroacetic acid extract, equivalent to 25 µL of serum, was analyzed in the same manner; 200 µL of distilled water then replaced the SLS.p 1401 : In footnote 1, the compound should read N 1-(α,α-dimethyl - β - hydroxyethyl) - 2- methyl - 1,2- propanediamine.p 1749 : In Table 3, column 4, row 2: "0-20" should read "0-2.0."p 1948 : In ref. 12, "Sud-" should read "Sul-" (publication is in Portugese, not Spanish).p 1952 : The names of two of the authors are misspelled: Cassani and Vanzetti. The correct spellings in the address are "Granda" and "Niguarda."Vol. 28p 156 : In Table 1, last column, delete "x" from the equations " y = 0.75 x " and " y = 1.71 x ."p 401 : In the address in the middle column, "State" should read "Safe."p 406 : Third column, under "Natelson Award," Normal should read Norman.p 557 : Just before the last sentence in the second paragraph in the middle column, insert the following sentence: "The exact degree of the enhancement depends highly on the amount of catalase impurity in the glucose oxidase preparation."

[学科分类] 过敏症与临床免疫学