Quantification of the Aminosteroidal Non-Depolarizing Neuromuscular Blocking Agents Rocuronium and Vecuronium in Plasma with Liquid Chromatography-Tandem Mass Spectroscopy

[摘要] Rocuronium (RO) and vecuronium (VE) are widely used aminosteroidal non-depolarizing neuromuscular blocking agents. There are few methods published for the determination of VE, its metabolite 3-desacetyl-vecuronium (OHV) (1)(2)(3)(4), and RO (5)(6) that use HPLC with ultraviolet, fluorescence, or electrochemical detection or gas chromatography with nitrogen-sensitive detection. To date, the methods published for the determination of VE and OHV use an exotic detection system (1), a time-consuming derivatization step (3), or a very laborious analytical technique (2)(4). The published methods for RO need a very sophisticated instrument that requires postseparation extraction of the drugs (6) or include a very time-consuming derivatization step (5). Despite the great analytical effort, the reproducibility (CV >10%) and detection limits (>10 μg/L) of these methods are not satisfying. To perform pharmacokinetic studies of RO, VE, and its metabolite OHV, we have established a robust, sensitive, and specific liquid chromatography electrospray ionization-tandem mass spectrometry method.Immediately after blood collection into heparin-containing tubes and centrifugation at 4 °C, 1 mL of plasma was added to a tube containing 0.2 mL of a 1 …

[学科分类] 过敏症与临床免疫学